Journal: PLoS ONE
Article Title: Systematic Functional Study of Cytochrome P450 2D6 Promoter Polymorphisms in the Chinese Han Population
doi: 10.1371/journal.pone.0057764
Figure Lengend Snippet: The relative luciferase activities were normalized against the human wild type construct. The results are the average of at least three independent experiments. Comparisons among groups were done with the aid of the ANOVA statistical procedure. *Significantly different from Wild type transfected cells (P<0.05). **Significantly different from Wild type transfected cells (P<0.01), ***Significantly different from Wild type transfected cells (P<0.001).
Article Snippet: The cells were incubated with the lipid-DNA complexes for 36 h. The culture medium was removed and washed with 500 ul PBS for each well, and the Renilla and Firefly luciferase activities were then detected using the Dual Luciferase reporter assay system kit (Glomax multi-detection system, Promega).
Techniques: Luciferase, Construct, Transfection